Flag antibody hrp
WebJan 1, 2010 · Horseradish peroxidase conjugated Mouse anti Histidine tag antibody clone AD1.1.0 (MCA1396P) ... As additional negative controls, two strips were incubated respectively with the anti-Flag antibody and the HRP-conjugated anti-mouse antibody, in order to monitor eventual non-specific signals due to the secondary antibodies used to … WebThe ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The …
Flag antibody hrp
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Web> 无血清快速细胞冻存液. QuickFreezing-M. QuickFreezing-2*M WebJun 6, 2016 · Introduction. Polypeptide tag technology, based on the interaction between a monoclonal antibody (mAb) and its epitope peptide, is an essential tool for protein analysis [1–4].Commercially available peptide tag systems such as the FLAG [5,6], HA [], MYC [], and V5 [] tags are widely used in cell biology and biochemical analysis of …
WebA FLAG tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, … WebA ~17 kDa band corresponding to HA-H3-FLAG were observed in HEK293E transfected lysates on probing with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1500 (Product # A44241).
WebI initially pulled down p53 using PAb421, and performed a Western Blot using an HRP-conjugated anti-FLAG antibody to detect if a certain POI was interacting with p53. WebFeb 2, 2014 · The membrane was probed with an HRP-conjugated HA Epitope Tag monoclonal antibody (Product # 26183-HRP) at a dilution of 1:1000 overnight at 4°C on a rocking platform and washed in TBS-0.1% …
WebApr 14, 2024 · The reacted samples were immunoblotted with an anti-streptavidin-HRP antibody. ... was transiently expressed in 293T cells and purified by immunoprecipitation …
WebAnti-FLAG-tag antibody (HRP) Catalog Number: orb705943: Species/Host: Rabbit: Conjugation: HRP: Tested applications: ICC, WB: Immunogen: KLH-conjugated synthetic … react pegasus trail 4 gore-tex donnaWeb3 hours ago · The detection was performed with an HRP-coupled anti-FLAG antibody. A significant signal could only be obtained for PSD95 PDZ1/2. (B) COS-7 cells were … react peg trail 4 gtx suWebTarget Background. GenScript THE™ DYKDDDDK Tag Antibody [HRP], mAb, Mouse is HRP conjugated THE™ Anti-DYKDDDDK Monoclonal Antibody (Mouse) (A00187) … react pegasus trail 4 sand driftWebDec 7, 2024 · OctA-Probe Antibody (H-5) is a mouse monoclonal IgG 1 κ OctA-Probe antibody, cited in 93 publications, provided at 200 µg/ml. raised against OctA (FLAG ® )-tagged proteins. OctA-Probe Antibody (H-5) is recommended for detection of OctA (FLAG®)-tagged fusion proteins of OctA and FLAG tagged proteins origin by WB, IP, IF … how to stay awake when you haven\u0027t sleptWeb3 hours ago · The detection was performed with an HRP-coupled anti-FLAG antibody. A significant signal could only be obtained for PSD95 PDZ1/2. (B) COS-7 cells were transfected with the indicated PDZ-domains fused to EGFP and a mitochondrial targeting sequence. After fixation with 4% PFA, the cells were stained with NbPSD95 coupled to … react performance benchmarkWebJan 15, 2016 · Catalog Number: A FLAG-tagged construct was expressed in HEK293T cells and 50 µg of clarified lysate was loaded for SDS-PAGe followed by Western Blotting with a FLAG-HRP conjugated antibody. The goal was to test this antibody so that one can exclude secondary HRP conjugated antibody step and save one hr of incubation. react pegasus trail 4 gtx - laufschuh trailWeb畅销产品. This APOO antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-38 amino acids from the N-terminal region of human APOO. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. react pegasus trail 4 running shoes womens