A typical solid phase extraction involves five basic steps. First, the cartridge is equilibrated with a non-polar or slightly polar solvent, which wets the surface and penetrates the bonded phase. Then water, or buffer of the same composition as the sample, is typically washed through the column to wet the silica surface. The sample is then added to the cartridge. As the sample pass… WebSolid-phase extraction (SPE) is a sample preparation technique that can be used for purification, enrichment/concentration, fractionation, or solvent ... If using cartridges, choose the proper size by (1) closely matching the sample volume to the cartridge volume and bed weight, and (2) closely matching the analyte ...
Do you really know SPE cartridge? And how to choose them?
WebMar 18, 2024 · Method for selecting SPE cartridge. The capacity of the SPE cartridge refers to the adsorption amount of the column packing. For silica-based solid-phase extraction columns, the capacity is generally 1 to 5 mg/100 mg, which means that the column capacity is 1% to 5% of the mass of the packing. The capacity of the bonded … WebTo assure compatibility, simply match sizes across parts when ordering. For example, if 6 mL SPE cartridges are used, then 6 mL SPE accessories are needed, so part numbers … gry o graffiti
Extraction Cartridges at Thomas Scientific
WebSep-Pak Accell Plus QMA Vac cartridges contain a silica-based, hydrophilic, strong anion-exchanger with large pore size (300Å). The cartridges are designed for extraction of anionic analytes in aqueous and non-aqueous solutions. The large pore size makes it ideal for isolation of larger…. Related Products: Sep Pak. Compare this item. WebWhen choosing a SPE cartridge, are you basing your choice on the right selection criteria? Now that we are talking about it, do you have any doubts? Take a look at the MythBusters capsule of the ... WebSep 29, 2024 · Principle of solid phase extraction cartridges. The basic principle of SPE cartridges is that the liquid sample is made to pass through the adsorbent to retain the tested substance. Then the impurities are washed away with the appropriate strength solvent. And then the tested substance is eluted with a small amount of good solvent, so … final fantasy 4 switch